PLAC8 promotes the autophagic activity and improves the growth priority of human trophoblast cells

Autophagy plays an important role in the normal development and function of trophoblast cells is precisely regulated during pregnancy. Dysregulated autophagy contributes to abnormal proliferation trophoblasts, which closely related occurrence pregnancy-related diseases. Placenta specific 8 (PLAC8, Onzin) a multifaceted protein proven promote potentiate various tumor progression. Its trophoblasts remains elusive. In our present study, PLAC8 expression was detected tissues first-trimester placentas (n = 5), term choriocarcinoma placental site trophoblastic 5). increased gestational neoplasms compared with pregnancies. mCherry-EGFP-LC3B reporter transmission electron microscopy confirmed promoted autophagic flux human cells. Both gain-of-function loss-of-function experiments demonstrated PLAC8-regulated autophagy-related genes, including ATG5, ATG12, Beclin-1. addition, data showed that co-localized p53 its degradation, re-expression partially abrogated PLAC8-induced activity. Furthermore, overexpression cell viability proliferation, acting as protective mechanism against cytotoxicity etoposide (VP-16). Such phenomenon effectively by inhibitors 3-methyladenine (3-MA) chloroquine (CQ). conclusion, trophoblasts. This study provided insights into significant for wide range diseases may contribute developing novel treatment strategies